Quinn seemed to be meant to be a fairly important character, and Puck was Finn's friend for a while, and then still collaborated with Finn. They became bros again. The football players were bros. This was Finn, Puck, Mike, Matt and later Sam and Artie. The circle of bros extended further to include Joe and Blaine. It took a while, but finally the other boys were convinced that Artie's physical limitations didn't stop him from being one of the boys. Blaine likes being around other boys in a homosocial environment. Puck said, "Even you, Blaine." Blaine passes (always that word with him) for performing gender in a mainstream way, at least on the surface. It's certainly arguable that Blaine doesn't actually perform gender in a stereotypically masculine (for the U.S.) way, when he's not trying to put on a performance. He's usually deliberately putting on a performance, though.
Kurt never became a bro. He likes being friends with girls, and aligned himself with them. I've seen it suggested that he felt safer with them, which was certainly a valid consideration. Eventually, though, Puck said that Kurt was his "boy." As much as the other boys at McKinley felt that Kurt queered gender with his voice, clothes, sexual orientation, and interests, he was eventually accepted as being a boy. Kurt told Burt, "I'm a guy, Dad." Kurt made it clear at least to Burt how he identified.
Tina was mainly in the background for quite a while, but this season she's "shouting to be heard," as some bloggers describe it. I like hearing her sing, and I like when she's calmly explaining things, but I take girls being rude much harder than I take boys being rude. I'm sure this is some internalized sexism, and socialization. But Tina is getting herself some attention.