Switching to the Western, on Glee, Kurt wears pieces of feminine clothing mixed in with masculine dress on a fairly regular basis. He also says and does gender non-conforming things with relative frequency. When I play songs on Glee CDs, both my Mom and S.M. say that he sounds like a woman. S.M. was a music teacher for many years. While one song was playing, I asked her if it would be considered sung in a soprano or alto. She said it had to be a tenor, because that was the male voice. "Maybe a bari-tenor," she said. But without visual reference, they think it's a woman's voice.
There was some discussion of body language between Biyuti and Larkin. Biyuti believes that Kurt's body language is pretty masculine -- in a non-heteronormative way. Blaine's body language is sometimes more femme. Blaine's never been seen wearing anything but masculine clothes, though. Kurt strikes me as an effeminate boy, though he seems to identify as male. RM (Racheline Maltese) has some very interesting things to say about gender for the two of them. Blaine is apparently supposed to be more straight-appearing, and in some ways he is. I think the suggestions that he might be more genderfluid than Kurt are quite interesting.